DELFIA® (dissociation-enhanced lanthanide fluorescence immunoassay) is a time-resolved fluorescence (TRF) intensity technology. Assays are designed to detect the presence of a compound or biomolecule using lanthanide chelate labeled reagents, separating unbound reagent using wash steps.
How does DELFIA work?
This immunoassay uses an antibody-coated plate to capture target analyte in the sample. The wells are then washed, and a DELFIA Europium-labeled antibody is added. After final washes, DELFIA Enhancement Solution is added to dissociate the Europium and allow it to form a new, highly fluorescent chelate in solution.
What is Alpha screen assay?
AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym “Alpha” stands for amplified luminescent proximity homogeneous assay.
What is AlphaLISA?
AlphaLISA is a bead-based luminescent amplification assay, offering greater sensitivity, a wider dynamic range and small sample sizes over traditional ELISA. In the AlphaLISA assay, a biotinylated antibody and an antibody-conjugated AlphaLISA Acceptor bead are used to capture the target analyte.
What is delfia? – Related Questions
What is TR FRET assay?
TR-FRET combines the time-resolved (TR) measurement of fluorescence with fluorescence resonance energy transfer (FRET) technology. In FRET assays, biomolecules (e.g., proteins) are labeled with donor and acceptor fluorophores. When the biomolecules interact, donor and acceptor fluorophores are brought close together.
What is time-resolved fluorescence?
Time-resolved fluorescence spectroscopy is a spectroscopy technique used to monitor interactions between molecules and motions that occur in the short periods. The ability to measure changes in the picosecond or nanosecond time range makes it a useful technique in biomolecular structure analysis and dynamics.
What are the three stages of fluorescence?
In short, the 3 steps of fluorescence are absorption (or excitation), non-radiative transition (or excited-state lifetime), and fluorescence emission.
Why is fluorescence measured at 90 degrees?
The detector is usually set at a 90-degree angle to the light source to avoid any interference from the transmitted excitation light.
What is a typical fluorescence lifetime?
The average time the electron stays in an excited state before photon emission is referred to as the lifetime. Fluorescence lifetime typically ranges in the order of 10− 9 to 10− 7 [s].
What makes a good fluorescence image?
Fluorescence imaging requires a delicate balancing act. On one hand, you need to acquire a high enough signal, which you can achieve by using a longer exposure time or stronger excitation light. On the other hand, you need to minimize phototoxicity for the sample condition by reducing the excitation time and intensity.
What is the range of fluorescence?
In general, fluorescence investigations are conducted with radiation having wavelengths ranging from the ultraviolet to the visible regions of the electromagnetic spectrum (250 to 700 nanometers).
What triggers fluorescence?
fluorescence, emission of electromagnetic radiation, usually visible light, caused by excitation of atoms in a material, which then reemit almost immediately (within about 10−8 seconds). The initial excitation is usually caused by absorption of energy from incident radiation or particles, such as X-rays or electrons.
What is fluorescence in simple terms?
Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. It is a form of luminescence.
What is the difference between fluorescence and fluorescent?
People often refer to lights for bringing out (exciting) fluorescence – whether for night diving or in the lab or field – as ‘fluorescent lights’. While there is no harm in that, they aren’t, and we try to be technically correct. ‘Fluorescent lights’ are what you commonly find in office buildings.
What color is fluorescent?
What Is Fluorescent Color? ‘Fluorescent’ refers to colors that absorb and reflect more light than conventional colors. Because of this, these pigments are brighter, bolder and better. Some people refer to fluorescent color as neon.
Why is it called fluorescent?
One of the first to explain it was the Irish scientist Sir George Stokes from the University of Cambridge in 1852, who named the phenomenon “fluorescence” after fluorite, a mineral many of whose samples glow strongly because of impurities.
How many colors are fluorescent?
Fluorescent or neon colors are extremely bright versions of primary and secondary colors, such as blue, red, green, yellow and purple. Fluorescent colors stand apart from other types of color due to the fact that they emit light, making them luminescent.
Is fluorescent better than LED?
Compared to fluorescent bulbs, LED lights are up to 80% more efficient. Unlike fluorescent lights, LED lights convert 95% of their energy into light and only 5% is wasted as heat; this is all while still using far less power to provide a strong and consistent output at a lower wattage.
Can I replace fluorescent tubes with LED?
Yes, you can replace fluorescent tubes with LED tubes or LED-integrated fixtures. If you just want to replace the bulbs, you can use plug-and-play, direct-wire, or hybrid LED tubes. Plug-and-play tubes are the easiest to install as they do not require any rewiring to the fixture.
Is it worth changing fluorescent to LED?
Replacing fluorescent tubes with LED lights is low-cost, energy-efficient lighting for you. The LED lights will give you better quality lighting and a much longer lifespan. Increased lifespan is one of the reasons LED lights have quickly become a popular alternative for fluorescent and incandescent lights.